TABLE 3.
Complementation of ΔpcuABCDE (strain 6611) and effects of single-gene deletions on the phenotypes
Activities of the mutant strains are expressed as a percentage of wild-type activity.
| Strain | Genes added for complementation | Relative cytochrome c oxidase activitya |
Relative Fix activitya | |
|---|---|---|---|---|
| Without 50 μm Cu(SO)4 | With 50 μm Cu(SO)4 | |||
| % | % | % | ||
| 6611 | 16.8 ± 4.7 | 100.1 ± 12.8 | 34.7 ± 13.5 | |
| 6611-32 | Only vector insert | 14.4 ± 5.1 | 90.3 ± 9.8 | NDb |
| 6611-33 | pcuABCDE+ | 106.6 ± 10.5 | ND | ND |
| 6611-6630 | pcuABCD[ΔE]+ | 113.4 ± 9.9 | ND | 112.8 ± 16.8 |
| 6611-1654 | pcuABC[ΔD]E+ | 120.5 ± 18.2 | ND | 127.8 ± 40.9 |
| 6611-34 | pcuAB[ΔC]DE+ | 24.8 ± 4.4 | 100.5 ± 10.1 | 36.1 ± 5.0 |
| 6611-1650 | pcuA[ΔB]CDE+ | 101.0 ± 17.0 | ND | 105.1 ± 8.5 |
| 6611-1653 | pcu[ΔA]BCDE+ | 111.8 ± 19.5 | ND | 99.7 ± 10.3 |
a The average wild-type cytochrome c oxidase activity (100%) is ∼0.31 μmol of horse heart cytochrome c oxidized/mg of membrane protein/min. The average wild-type Fix activity (100%) is 2.82 ± 0.4% C2H4/min/g.
b ND, not determined.