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. 2012 Sep 20;287(46):38913–38921. doi: 10.1074/jbc.M112.388389

FIGURE 2.

FIGURE 2.

Radiation induces expression of TGIF1 and proinflammatory genes in vitro in endothelial cells. A, mRNA levels of TGIF1 3, 6, 24, 48 and 72 h after 10 Gy. B, representative Western blot (IB) of TGIF1 protein expression 24 h after 10 Gy. Relative quantification was performed from four experiments performed in triplicate. C, mRNA levels of IL-6, IL-8, CXCL1, MIP-2, and MCP-1 in HUVECs 3 h after 10 Gy. mRNA levels were calculated relative to GAPDH as housekeeping gene and normalized to a value of 1 for nonirradiated cells. Results are the mean ± S.E. (error bars) of three independent experiments done in triplicate. *, p < 0.05 versus nonirradiated cells.