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. 2012 Sep 18;287(46):38922–38935. doi: 10.1074/jbc.M112.365148

FIGURE 5.

FIGURE 5.

Oral administration of gemfibrozil up-regulates TPP1 in vivo in the hippocampus of WT and PPARβ−/− but not PPARα−/− mice. WT, PPARα−/−, and PPARβ−/− mice (n = 4 in each group) were treated with 7.5 mg/kg body weight/day of gemfibrozil (dissolved in 0.1% methylcellulose) or vehicle (0.1% methylcellulose) via gavage. After 21 days of treatment, mice were killed, and hippocampal (CA1 and dentate gyrus) sections were double-labeled for TPP1 (red) and GFAP (green). DAPI was used to visualize the nucleus. A–C, TPP1 levels were compared between the dentate gyrus region of vehicle-treated and gemfibrozil-treated WT (A), PPARα−/− (B), and PPARβ−/− (C) mice. Scale bar, 20 μm. D, higher magnification images showing co-localization of TPP1 and GFAP in the dentate gyrus of gemfibrozil-treated mice (WT, PPARα−/−, and PPARβ−/−). Scale bar, 10 μm. E–G, TPP1 levels were compared between the CA1 region of vehicle-treated and gemfibrozil-treated WT (E), PPARα−/− (F), and PPARβ−/− (G) mice. Scale bar, 20 μm. H, higher magnification images showing co-localization of TPP1 and GFAP in the CA1 region of gemfibrozil-treated mice (WT, PPARα−/−, and PPARβ−/−). Scale bar, 10 μm. All results represent analysis of each of three hippocampal sections of each of four different mice per group.