FIGURE 6.

Change in ROS accumulation in the presence of various concentrations of D737. DCF fluorescence of cells incubated with water as a positive control was normalized to 100% (light gray bars). DCF fluorescence above 100% indicates increased accumulation of ROS. a, D737 did not significantly affect ROS accumulation induced by hydrogen peroxide. PC12 cells were incubated for 24 h with 200 μm H₂O₂ (dark gray bar) or D737 and 200 μm H₂O₂ (hatched gray bar). b, D737 reduced the ROS accumulation induced by Aβ42. PC12 cells were incubated for 24 h with 20 μm Aβ42 (black bar) or Aβ42 and various concentrations of D737 (hatched black bars). a.u., absorbance units.