FIGURE 2.

CXCL12-induced expression of SHH is mediated through CXCR4 in pancreatic cancer cells. MiaPaCa and Colo357 cells were transiently transfected with non-target (NT) or CXCR4- and CXCR7-targeted siRNAs. Following 24 h of transfection, cells were treated with CXCL12 (100 ng/ml) for another 24 h period. Total protein was isolated and subjected to immunoblot analysis to assess the expression of CXCR4, CXCR7, and SHH. β-actin was used as an internal control. Data indicate specific silencing of CXCR4 and CXCR7 by target-specific siRNAs, and clearly demonstrate that CXCL12-induced SHH expression is specifically mediated through CXCR4 in pancreatic cancer cells.