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. 2012 Sep 20;287(46):39205–39215. doi: 10.1074/jbc.M112.376053

FIGURE 4.

FIGURE 4.

Rescue of function experiments of truncated GlyRα1 variants. A, representative traces of cells transfected with α1-trc and truncated tail variants (Myc-iDΔx-TM4). Maximal glycine-gated currents were determined following 1 mm Gly application in a whole-cell configuration of double-transfected HEK293 cells. Recordings were carried out at −60 mV, [Cl−]in = [Cl−]out. Agonists were applied via a U-tube for 1 s. The arrows point toward the appropriate rescue combinations of α1-trc together with different complementation constructs B, bar diagram of the rescue efficiencies determined by the mean Imax values with error bars representing S.E. The rescue efficiency in a three-domain GlyR configuration is emphasized with a black frame. When the TM3-4 loop(357–418) (3–4loop) was added as a separate expression construct to a nonfunctional two-domain configuration (α1-trc and Myc-iDΔ62-TM4), receptor function was again restored, although with low efficiency (compare α1-trc + Myc-iDΔ62-TM4 with α1-trc + Myc-iDΔ62-TM4 + TM3-4 loop(357–418)).