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. 2012 Sep 19;287(46):39233–39244. doi: 10.1074/jbc.M112.369975

FIGURE 6.

FIGURE 6.

Role of XRCC1 and the interaction between XRCC1 and PNKP in the recruitment and retention of EYFP-PNKP to sites of laser-induced DNA damage. A, representative cells showing the recruitment and retention EYFP-PNKP to sites of laser-induced DNA damage (red box) in xrcc1 mutant EM9 cells (upper panel), EM9 cells stably expressing Xpress-tagged A482T XRCC1 (XR1PNK482, middle panel), and EM9 cells stably expressing Xpress-tagged wild-type XRCC1 (XR1WT, lower panel). B, initial recruitment of EYFP-PNKP to the damaged site in EM9 cells expressing no (Z, top), the A482T mutant (PNK, middle), or wild-type (WT, bottom) XRCC1 protein. C, retention of EYFP-PNKP at the damaged site (see panel B). The results of 10 to 15 independent cells from the three cell lines are shown graphically in panels B and C.