FIGURE 7.
Comparison of the recruitment and retention of wild-type XRCC1 and the A482T mutant to sites of laser-induced DNA damage. A, representative cells showing the recruitment and retention of wild-type XRCC1 (XR1WT, upper panel) and the A482T mutant version (XR1PNK482, lower panel) fused to EYFP to sites of laser-induced DNA damage (red box) in HeLa cells. B, initial recruitment of the wild-type or A482T mutant EYFP-XRCC1 fusion proteins to the damaged site. C, retention of the wild-type or A482T mutant EYFP-XRCC1 fusion proteins at the damaged site. The results of 9 or 10 independent cells transfected with either wild-type XRCC1 or the A482T mutant of XRCC1 are shown graphically in panels B and C.