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Kinetic parameters for PNKP; effect of DNA ligase IIIα−XRCC1
kcat values were calculated using the equation Kcat = Vmax/ [E], where E is PNKP concentration.
| Reaction | km | kcat | kcat/km |
|---|---|---|---|
| nm | min−1 | nm−1 min−1 | |
| PNKP only | 17.9 ± 1.4 | 25.3 ± 0.9 | 1.4 |
| PNKP plus XRCC1-ligase III | 10.1 ± 1.1 | 32.8 ± 2.1 | 3.2 |
