FIGURE 4.

siRNA knockdown of CLASP2 in L6 myotubes reduces insulin-stimulated localization of GLUT4 to plasma membrane ruffles. A, siRNA knockdown of CLASP2 in L6 myotubes was performed as described under “Experimental Procedures.” B, L6 myotubes were serum-starved and either left untreated or treated with 10 nm insulin for 15 min. Confocal imaging was performed as described under “Experimental Procedures.” C, effect of siRNA knockdown of CLASP2 on the percentage of membrane ridges with GLUT4 after insulin stimulation, performed as described under “Experimental Procedures,” is quantified. D, effect of siRNA knockdown of CLASP2 on the density of GLUT4 per membrane ridge after insulin stimulation, performed as described under “Experimental Procedures,” is quantified. Error bars, S.E. *, p < 0.05.