Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Nov 9;3:247. doi: 10.3389/fpls.2012.00247

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © Klein, Seidel, Stöcker and Dietz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

PMC Copyright notice

Subcellular localization of ANAC089 in A. thaliana protoplasts. (A) Protoplast in bright field. Fluorescence image of a protoplast expressing the C-terminally CFP-tagged ANAC089 (B) or the C-terminally YFP-tagged ANAC089 (C). Comparative localization of ANAC089 und ABI5 with C-terminally fused fluorophores in A. thaliana protoplasts. (D) Bright field control picture. (E) ANAC089-CFP-signal detected in vesicles, endoplasmic reticulum, and plasmamembrane. (F) ABI5-YFP-signal in cell nucleus. (G) Dimerization detection by FRET analysis: protoplasts were co-transfected with either CFP:ANAC089 and YFP:ANAC089:YFP (left column) or ANAC089:CFP and ANAC089:YFP (right). In both cases significant FRET above the threshold of co-expressed free CFP and YFP could be detected (mean ± SD, n > 60 protoplasts, three transfections). (H) Putative topology of ANAC089 in the membrane.