FIGURE 6.

Co-localization of fluorophore-fused ANAC089 with marker proteins. Protoplasts were co-transfected with ANAC089 fused to either CFP or YFP as indicated and a marker protein as follows: (A–C) AHA1, the plasmamembrane H⁺-ATPase, (D–F) VHA-e, a subunit of the V₀ domain of V-ATPase that localizes to TGN/EE, (G–I) VMA21a, an ER-resident assembly factor of the V-ATPas, (K–M) Clathrin, the light chain of the clathrin coat, (N–P) Sar1, the small GTPase of COP II vesicle transport anterograde transport between ER and Golgi, and (Q–S) GOS12, a Golgi SNARE.