Figure 3.

a) In the 4-month-old rat cerebellum, in the most rostral and caudal lobules, no immunoreaction product is present in the Purkinje cell layer; b,c) representative photographs of portions of the most rostral and caudal cerebellar lobules in 28-month-old rats. Punctate immunoreaction product is visible at the apical pole of Purkinje cells; d) particular of the most caudal cerebellar lobules in a section immunostained for calbindin from 28-month-old rat. The cytoarchitecture is apparently well preserved. ML, molecular layer; Pc, Purkinje cell layer; IGL, granule cell layer. Scale bar a,b,c: 20 µm; d: 50 µm.