Figure 1.

Western blot of crude protein extract (left panel) from the central brain mass of Octopus vulgaris revealed using peroxidase labeled pre-immune serum (left column) and peroxidase labeled cChAT (right column). A clear band at approximately 81-kDa (arrowhead) is recognized by cChAT antiserum. Molecular weight marker is Bench Mark Prestained Protein Ladder (Invitrogen, Carlsbad, CA, USA). cChAT immunoreactivity in the octopus pedal lobe (right panel) using cChAT antiserum (a) or cChAT antiserum pre-absorbed with antigenic peptide (b). Scale bar: 30 µm.