Figure 3.
(A) Predicted secondary structure of the bovine blood–brain barrier (BBB) GLUT1 glucose transporter is shown, which is formed by 12 transmembrane domains, a glycosylated extracellular loop between transmembrane domains 1 and 2, and intracellular amino and carboxyl termini.128 (B) Northern blot of mRNA derived from either total rabbit brain (lane 1) or capillary-depleted rabbit brain (lane 2) for either the GLUT1 glucose transporter or actin.71 (C) Electron microscopic immunogold study of human brain with a primary antiserum against the purified human erythrocyte glucose transporter, and a secondary antibody conjugated with 10 nm gold particles, shows abundant expression of immunoreactive GLUT1 glucose transporter on the luminal (L) and abluminal (AL) membranes of the capillary endothelium in human brain.70