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. 2012 Aug 29;32(11):1959–1972. doi: 10.1038/jcbfm.2012.126

Figure 6.

Figure 6

(A) Conjugate of an IgG–avidin fusion protein and a monobiotinylated peptide radiopharmaceutical. The biotin group on the peptide binds to the avidin domain of the fusion protein to form a high affinity linkage between the IgG molecular Trojan horse and the peptide radiopharmaceutical. (B, C) Scans of Rhesus monkey brain at 3 hours after the intravenous (IV) injection of either [125I, N-biotinyl]-Aβ1–40 alone (B), or [125I, N-biotinyl]-Aβ1–40 conjugated to the HIRMAb (monoclonal antibody) molecular Trojan horse via a streptavidin linker (C).115 (D) A rat brain tumor model was produced by the intracerebral injection of human U87 glioma cells in nude rats. Immunocytochemistry (ICC) of brain removed 16 days after tumor implantation with an antibody to the human EGFR shows abundant expression of the EGFR in the brain tumor.119 (E) Scan of rat brain removed 2 hours after the IV injection of [111 In-DTPA, Lys-biotinyl]–EGF conjugated to the TfRMAb molecular Trojan horse via a streptavidin linker. The section used for the film autoradiography was parallel to the section used for the ICC study in (D).119