Figure 2. (A–C) Expression of FV (A), FX (B) and FVIII (C) by eight human primary cell types: stem cells, fibroblasts, endothelial cells, macrophages, preadipocytes and adipocytes, hepatocytes and monocytes, with and without exposure to LPS.

The level of each mRNA was measured in triplicate and error bars show standard deviations of means for triplicate cultures per condition and is represented as the mean ± SEM. The p-value obtained from a paired t-test with a two-tailed test samples (T-test). P < 0.05 is considered statistically significant. (A) shows the expression of the FV gene by human monocytes (dark-gray bar) and hepatocytes (light-gray bar). Monocytes and hepatocytes were not exposed to LPS. Hepatocytes were used as a positive control for coagulation factor expression. Human primary hepatocytes die upon exposure to LPS, and monocytes were not exposed to LPS because LPS treatment results in the differentiation of monocytes into macrophages. mRNA expression is expressed as ²log (logarithm base 2) values on the y-axis. Relative mRNA intensity was obtained from three measurements and is represented as the mean ± SEM. (B) shows the expression of the FX gene by human fibroblasts, preadipocytes, adipocytes and hepatocytes. Gene expression is expressed as ²log values on the y-axis. Relative mRNA intensity was obtained from triplicate measurements and is represented as the mean ± SEM. Hepatocytes served as a positive control for coagulation factor expression. (C) shows the expression of the FVIII gene by human primary cells. FVIII mRNA expression was expressed as²log values on the y-axis. FVIII mRNA expression was observed in all cells tested except endothelial cells. Relative mRNA intensity was obtained from three measurements and is represented as the mean ± SEM. Hepatocytes served as a positive control for coagulation factor expression.