Fig. 2.

Alignment of the deduced amino acid sequences of capsanthin-capsorubin synthase (LlCCS) from L. lancifolium (GenBank accession No. JF304153), capsanthin-capsorubin synthase (CaCCS) from C. annuum (GenBank accession No. Q42435.1) and lycopene β-cyclase (LlLCYB) from L. lancifolium (GenBank accession No. GU471230). White letters on a black background indicate globally conserved residues; black letters on a light-gray background indicate identical residues, and white letters on a dark-gray background indicate similar residues. Dashes represent gaps introduced to improve the alignment. The putative cleavage site of the N-terminal plastid transit peptide is boxed. The position of the heterologous non-degenerate primer (ccs-3Rc) used in 3′RACE of the Llccs is indicated by a dashed arrow. The conserved FLEET motif essential for β- and κ-cyclase activity is underlined. Black triangles designate conserved amino acids with important catalytic activity as identified by Mialoundama et al. (2010).