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. 2012 Sep 24;53(11):1899–1912. doi: 10.1093/pcp/pcs128

Table 1.

The primers used for cloning and expression analysis by RT–PCR of genes for lycopene β-cyclase (LllcyB) and capsanthin-capsorubin synthase (Llccs) from Lilium lancifolium

Primer Sequence (5′ → 3′)
Cs-F TGGAGTTTGGGTTGATGAGT
Cs-R TGGGAATCTCTCCAATCCAT
ccs-3Rc TCTGGATAAGATGCTCTTCATGGATTGGAG
ccs-In ATGCCAATACCTCGGTTCCAGA
ccs-Out CTCTTCCAAGACGCATGAGCAA
Llccs-F GGATCCACCGTCCAGCCGCCTCCTTG
Llccs-R GGATCCATGTGAATTGCATTATATAA
lcyB-3Rc AGAAGAGACTTCTTTGGTTGCTC
lcyB-In TCTCCAATCCATGAAGAGCATCTTATCCAG
lcyB-Out GCCGAGCAACCAAAGAAG
P-ccs-R1 CAGCGGACCTCCCATTGGAAACAAACACTT
P-ccs-R2 TTGGAATAGCAGCGTTGTGAGCACGGTGTT
P-ccs-F1 ATTGGCATGGGTTTATGTCGTCGAGGCTGT
P-ccs-F2 GATGTTGTGACCAAGTGCCCTTTGCCTTTG
L-Llccs-F GCAGCAAACCATGAACCTTTG
L-Llccs-R ATTAAGATATGTTTCGTGAT
E-Llccs-F CTCCTCCGCCCACCGCTAC
E-Llccs-R CGGAGCAGGAGACGGAGGAG
E-LllcyB-F GGGATTCTCGCTGAGGTGGAA
E-LllcyB-R CCCAAAAGGCCATGGTCAGA
E-LhAct-F ATGTATGTTGCAATCCAGGCTGTGC
E-LhAct-R ATACCAGTAGCTTCCATTCCAACCA