Figure 1. Phenotypic characterization of Rb^F/F;K14creER^TM;p107^−/− mice.

a,b) Example of gross appearance of the Rb^F/F;p107^−/− (a) and Rb^F/F;K14creER^TM;p107^−/− (b) mice 4 months after topical tamoxifen treatment. Macroscopic aspect of face, dewlap, snout and eyelid (c, c',c'' respectively). d–f) H&E stained snout sections of Rb^F/F;p107^−/− (d) Rb^F/F;K14creER^TM (e) and Rb^F/F;K14creER^TM;p107^−/− (f) showing massive hyperplasia, hyperkeratosis and epithelial downgrowths in Rb^F/F;K14creER^TM;p107^−/−. g, g') Immunofluorescence showing the localization of Laminin in hyperplasic (g) and lesional areas (g') of Rb^F/F;K14creER^TM;p107^−/− mouse snouts. The reduced expression and disappearance of laminin in lesional areas support the invasive consition of squamous cell carcinomas. h–m) H&E stained sections showing tumor samples of snout (h, i), neck (h'), eyelid (h''), lip (j), palate (k), oral epithelium (l) and ventral tongue (m) of Rb^F/F;K14creER^TM;p107^−/− mice 4 months after tamoxifen treatment. n) Kaplan Meier plot showing the incidence of tumours in Rb^F/F;K14creER^TM (open box; n = 25) and Rb^F/F;K14creER^TM;p107^−/− (black box; n = 22) mice. p value was obtained by the log rank test. Bars = 150 µm.