Fig. 2.

Effects of tetrandrine citrate on the growth of CML cells and normal blood cells

(a) Cell viabilities of the indicated CML cell lines, primary CML cells, and normal blood cells (N) measured by MTT assay. Cells were incubated with tetrandrine citrate at a range of concentrations for 48 h; (b) Average percent of CFUs formed after primary CML cells were seeded at a low cell density and incubated with tetrandrine citrate (0, 2.0, or 6.0 μg/ml) for 14 d. The averages are normalized to untreated control (100%); (c) Untreated or tetrandrine citrate-treated (2.0 μg/ml, 14 d) primary CML cells immunofluorescently stained with anti-CD34⁺ antibodies (red) or stained with DAPI (blue)