Skip to main content
. 2012 Jun 6;4(8):705–718. doi: 10.1002/emmm.201101105

Figure 2. Lethality of female Igf2 +m/−p progeny derived from Igf2 +m/−p, p53 +/− fathers is associated with reduced lung growth.

Figure 2

  1. Male (n = 115) and female (n = 87) progeny at P10 from the 129 Igf2+m/−p, p53+/− inter-cross did not segregate according to the expected Mendelian distribution (see Table 1; p < 0.05) and were significantly different to expected numbers estimated from previous litter sizes (total expected numbers: males = 175, females = 175 based on mean litter size from earlier 129 breeding, p < 0.0001, χ2-test). Solid bars, observed numbers, grey unfilled bars, expected numbers (see also Table 2).
  2. Litter of 13 pups delivered by caesarean section at E19.5 from a WT female mated with Igf2+m/−p, p53+/− male. One Igf2+m/−p female (white arrow) became increasingly cyanotic and died 1hr post-delivery. The remaining four Igf2+m/−p females (thick black arrows) were also cyanotic compared to their littermates (thin black arrow points to a representative Igf2+m/−p male).
  3. Lung weights from E18.5 Igf2+m/−p female progeny derived from Igf2+m/−p, p53+/− fathers were significantly lower than those from Igf2+m/−p male progeny (*p = 0.025), WT female progeny (***p < 0.0001) and Igf2+m/−p, p53+/− female progeny (*p = 0.049, two-tailed t-test, NS = not significant).