Table 2.
Complete perinatal lethality of 129J Igf2+m/−p female progeny was only observed from females mated with 129J Igf2+m/−p, p53+/− males.
| Cross | Time-point | WT | Igf2+m/−p | p53+/− | Igf2+m/−p, p53+/− | p53−/− | Igf2+m/−p, p53−/− | Total | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p-Value | ||||||||||||||
| χ2-Value | ||||||||||||||
| ♂ | ♀ | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ | ♂ | ♀ | |||
| WT ♀ x | P0 | 74 | 82 | 54 | 59 | – | – | – | – | – | – | – | – | 269 |
| Igf2+m/−p ♂ | (67) | (67) | (67) | (67) | p = NS | |||||||||
| 0.67 | 3.23 | 2.61 | 1.01 | 7.54 | ||||||||||
| P3.5 | 66 | 77 | 32 | 35 | – | – | – | – | – | – | – | – | 210 | |
| (53) | (53) | (53) | (53) | p < 0.0001 | ||||||||||
| 0.35 | 11.4 | 8.00 | 5.83 | 28.74 | ||||||||||
| p53+/− ♀ x | P10 | 20 | 18 | 13 | 18 | 16 | 22 | 13 | 17 | – | – | – | – | 137 |
| Igf2+m/−p ♂ | (17) | (17) | (17) | (17) | (17) | (17) | (17) | (17) | p = NS | |||||
| 0.48 | 0.04 | 0.99 | 0.04 | 0.99 | 1.39 | 0.07 | 0.00 | 4.02 | ||||||
| WT ♀ x | E18.5 | 7 | 3 | 7 | 4 | 6 | 1 | 6 | 3 | – | – | – | – | 37 |
| Igf2+m/−p, p53+/− ♂ | (5) | (5) | (5) | (5) | (5) | (5) | (5) | (5) | p = NS | |||||
| 1.22 | 0.57 | 1.22 | 0.08 | 0.48 | 2.84 | 0.48 | 0.57 | 7.32 | ||||||
| P0 | 9 | 9 | 5 | 0 | 8 | 8 | 9 | 2 | 50 p < 0.05 | |||||
| (6.3) | (6.3) | (6.3) | (6.3) | (6.3) | (6.3) | (6.3) | (6.3) | 14.00 | ||||||
| 1.21 | 1.21 | 0.25 | 6.25 | 0.49 | 0.49 | 1.21 | 2.89 | |||||||
| P10 | 6 | 12 | 4 | 0 | 6 | 13 | 6 | 5 | – | – | – | – | 52 | |
| (6.5) | (6.5) | (6.5) | (6.5) | (6.5) | (6.5) | (6.5) | (6.5) | p < 0.01 | ||||||
| 0.04 | 4.65 | 0.96 | 6.5 | 0.04 | 6.5 | 0.04 | 0.37 | 19.08 | ||||||
| p53+/− ♀ x | E9.5 | 6 | 7 | 4 | 4 | 10 | 15 | 9 | 11 | 2 | 3 | 4 | 5 | 80 |
| Igf2+m/−p, p53+/− ♂ | (5) | (5) | (5) | (5) | (10) | (10) | (10) | (10) | (5) | (5) | (5) | (5) | p = NS | |
| 0.20 | 0.80 | 0.20 | 0.20 | 0.00 | 2.50 | 0.10 | 0.10 | 1.80 | 0.80 | 0.20 | 0.00 | 4.40 | ||
| E18.5 | 3 | 2 | 1 | 3 | 5 | 7 | 5 | 4 | 3 | 2 | 2 | 3 | 40 | |
| (2.5) | (2.5) | (2.5) | (2.5) | (5) | (5) | (5) | (5) | (2.5) | (2.5) | (2.5) | (2.5) | p = NS | ||
| 0.10 | 0.10 | 0.90 | 0.10 | 0.00 | 0.80 | 0.00 | 0.20 | 0.10 | 0.10 | 0.10 | 0.10 | 1.40 | ||
| P10 | 10 | 8 | 7 | 0 | 15 | 12 | 10 | 10 | 2 | 3 | 1 | 1 | 75 | |
| (4.7) | (4.7) | (4.7) | (4.7) | (9.4) | (9.4) | (9.4) | (9.4) | (4.7) | (4.7) | (4.7) | (4.7) | p = NS † | ||
| 6.02 | 2.34 | 1.14 | 4.7 | 3.38 | 0.74 | 0.04 | 0.04 | 1.54 | 0.61 | 2.90 | 2.90 | 12.487 | ||
Significant deviation from the normal expected Mendelian ratio of Igf2+m/−p progeny from WT mothers at P3.5 in both females and males was detected, suggesting an effect of the paternal allele on survival. The Igf2+m/−p genotyped progeny derived from Igf2+/−, p53+/− males were absent at P10 compared to E18.5, although the significance was not detected in the segregation of genotypes in progeny derived from p53+/− females (†).