Mating strategy of dARKO/Pten+/− mouse generation.
Genotyping results of Wt-AR/Pten+/− and dARKO/Pten+/− mice by using 2–9/select, FSP1-cre, Tgln-cre and Pten primer sets. Tail genomic DNA was isolated from Wt-AR/Pten+/− and dARKO/Pten+/− mice and PCR genotyping was performed to identify Wt and deleted AR fragment, FSP1-cre, Tgln-cre and Pten alleles.
DLPs from 9-month-old Wt-AR/Pten+/+, Wt-AR/Pten+/− and dARKO/Pten+/− mice were subjected to AR IHC staining. Arrows indicate the AR immunoreactive stromal cells and arrowheads represent the AR negative stromal cells. Epithelium from three different genotypes of mice all showed AR positive expressions. Data are presented as mean ± SEM. N = 5 mice per group. Scale Bars = 200 µm (400×) and 50 µm (1000×).
Loss of stromal fibromuscular AR reduced prostate size in APs and DLPS of Pten+/− mice. The representative gross appearance from 9-month-old Wt-AR/Pten+/+, Wt-AR/Pten+/− and dARKO/Pten+/− mouse prostates.
Serum testosterone was monitored by collecting the serum from 9-month-old Wt-AR/Pten+/− and dARKO/Pten+/− mice. The testosterone ELISA kit was used per the manufacturer's instructions. Data are presented as mean ± SEM. N = 5 mice per group.
