Figure 3. Stromal fibromuscular AR regulates epithelium proliferation, ECM remodelling, neovasculature formation and immune cell infiltration in Pten deficient mice.

N = 6–7 mice per group. Scale Bars = 200 µm (100×) and 50 µm (400×). The quantification results are in the right panels of each figure.

1. BrdU staining was used to evaluate proliferative cells. Arrows indicate BrdU positive cells.
2. Wt-AR/Pten^+/+, Wt-AR/Pten^+/− and dARKO/Pten^+/− mouse DLPs were subjected to Masson's Trichrome staining to measure deposited collagen fibers that stain blue colors (asterisks).
3. CD31 IHC staining was used to identify endothelial cells. Arrows indicate CD31+ endothelial cells.
4. CD3 and F4/80 IHC staining of DLPs from three genotypes of mice were used to characterize infiltrated T-cells and macrophages, respectively. Arrows indicate CD3+ staining T-cells (upper panel) and F4/80+ macrophages (lower panel).