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. 2012 Jun 18;4(8):825–839. doi: 10.1002/emmm.201101127

Figure 4. MITF-A binds the Tgfa promoter.

Figure 4

  1. Electrophoretic mobility shift assay (EMSA) using a specific mouse 32P-labelled TGF-α E probe and nuclear protein extracts from MITF-A transfected CTAL cells. Complexes were surpershifted with an anti-MITF antibody.
  2. EMSA using a specific mouse 32P-labelled TGF-α E probe and nuclear extracts from mouse kidneys.
  3. Chromatin immunoprecipitation assay in cells transfected with the empty or the FLAG-MITF-A expression vector.
  4. Luciferase assay of CTAL cells transfected with the wild type or the E box-mutated TGF-α reporter vector together with the empty or MITF-A expression vector. Data are means ± SEM; n = 4–6.