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. Author manuscript; available in PMC: 2013 Oct 31.
Published in final edited form as: J Am Chem Soc. 2012 Oct 19;134(43):17900–17903. doi: 10.1021/ja3090498

Figure 4.

Figure 4

Reconstitution of CazE and CazF activity in vitro. (A) HPLC analysis (280 nm) of α-pyrones biosynthesized by CazF in the presence of (i) malonyl-CoA and (ii) malonyl-CoA and SAM. (B) HPLC analysis (360 nm) of chaetoviridins enzymatically synthesized in vitro when (i) CazF was incubated with 8, malonyl-CoA, NADPH and SAM; (ii) CazE was incubated with 8, malonyl-CoA, NADPH and SAM; (iii) CazE and CazF were incubated with malonyl-CoA; (iv) CazE and CazF were incubated with malonyl-CoA and SAM; (v) CazE and CazF were incubated with malonyl-CoA and NADPH; (vi) CazE and CazF were incubated with malonyl-CoA, NADPH and SAM.