Fig. 6.

Noncanonical start codon usage at locus Medtr4g034810 of M. truncatula. Panel (A) shows a graphical depiction of the locus at two levels of resolution. Shown in each sub-panel are length of depicted region (in base pairs), chromosome ID, location of mapped EPTs, the Mt3.5v4 gene model and frame translation (in secondary panel), and depth of RNA-Seq coverage (scale in reads per bp shown at left). EPT_054593 (9 PSMs) maps to a genomic location upstream of the annotated start codon. Note the optimal sequence context at positions −3 and +4 relative to the proposed start codon. Panel (B) shows a multiple alignment of the protein sequences between the putative ribosomal protein S8 encoded by Medtr4g034810.1 (using the proposed noncanonical start codon) and its closest homolog in Arabidopsis which uses a canonical ATG start codon.