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. 2012 Jul 3;11(10):916–932. doi: 10.1074/mcp.M111.015370

Fig. 4.

Fig. 4.

PAK2 contributes to cell proliferation, migration and invasion. A, Immunoblot of H1299 cells 4 days after infection with shRNA control virus (Cont) or with shRNA directed against PAK2 (Sh1). B, H1299 cell line growth curve after shRNA infection. Cell viability was measured using the WST-1 reagent for 4 days after seeding the cells in the culture plate. Solid line - control virus; Dashed line - PAK2 shRNA containing virus C, Colony formation assay using H1299 cells seeded 4 days after infection control virus (Cont) or PAK2 shRNA containing virus. (Sh1) D, Transmembrane migration assay results with IPA-3 at the indicated concentrations. E, Matrigel invasion assay results with IPA-3 at the indicated concentrations.