Table I. Quantification of SIRT1-modulated Lys acetylation in known acetyltransferases, including Ep300, Crebbp, Ncoa3, Hat1, Kat14 (Csrp2bp), Kat5, Kat8 (Myst1), Kat7 (Myst2), Kat6a (Myst3), and Kat6b (Myst4).

“Known site” refers to Kac sites previously identified in either human or mouse based on the Uniprot (http://www.uniprot.org) and PhosphoSite (http://www.phosphosite.org) databases. Kat# for acetyltransferases are based on published nomenclature for chromatin-modifying enzymes (66). * Kac sites with Mascot scores below 20.0.

Acetyltransferases	Gene name/Kat#	Acetylation position	Ratio KO/WT	Known site?
Nuclear receptor coactivator 3	Ncoa3/Ncoa3	608	8.42	Yes
		611	8.06	Yes
		612	2.62	Yes
Histone acetyltransferase type B catalytic subunit	Hat1/Hat1	6	2.48
Cysteine-rich protein 2-binding protein	Csrp2bp/Kat14	275	KO only
Histone acetyltransferase KAT5	Kat5/Kat5	85	8.38	Yes
MYST histone acetyltransferase 1	Myst1/Kat8	113*	15.61	Yes
		154	17.7
		168	8.16
		351	0.94
		410*	2.31
MYST histone acetyltransferase 2	Myst2/Kat7	157	1.88	Yes
		201	13.76
		223	10.17
		279*	KO only
		282*	KO only
MYST histone acetyltransferase 3	Myst3/Kat6a	94	2.47
		355	0.75	Yes
		406	KO only
		414	KO only	Yes
		813	1.23
		816	1.23	Yes
		840	8.85
MYST histone acetyltransferase 4	Myst4/Kat6b	380	2.91
		394	2.91
		433	KO only
		441	KO only	Yes
E1A binding protein p300	Ep300/Ep300	351*	5.39	Yes
		637*	1.98	Yes
		1179	0.96	Yes
		1541	1.43	Yes
		1545	1.55	Yes
		1553	1.57	Yes
		1554	1.57	Yes
		1557	1.44	Yes
		1559	1.57	Yes
		1589	1.01	Yes
		1673	1.55	Yes
CREB binding protein	Crebbp/Crebbp	365*	5.39
		1217	1.19	Yes
		1584	1.9	Yes
		1587	1.9	Yes
		1592	2.54	Yes
		1593	2.48	Yes
		1596	2.48	Yes
		1598	2.48	Yes
		1621	1.01
		1628	1.01	Yes
		1712	1.55	Yes
		1742	2.52	Yes
		1745	0.79	Yes
		1938	1.18
		2092	KO only