Table I. Comparison of predicted protein association scores with experimental values of TG2 activity (normalized FI) and interaction (Kd).
Confidence scores were obtained from the STRING database. TG2 activities of the TG2-related proteins were normalized by median-centering. N.D., nondetectable; AI grade, activity-interaction grade; A, activity; H, high; L, low; I, interaction; S, strong; W, weak; N, non-detectable.
| Protein | Confidence score | Integrative proteomic profiling |
||
|---|---|---|---|---|
| TG2 activity (normalized) | TG2 interaction Kd (nM) | AI grade | ||
| Fibronectin | 0.996 | 4.199 | 6.44 | AHIS |
| Annexin A1 | 0.747 | 0.396 | 6.99 | ALIS |
| Grancalcin | 0.708 | 0.453 | 3.82 | ALIS |
| G-actina | 0.688 | 4.012 | 25.42 | AHIW |
| E-cadherin | 0.659 | 5.653 | N.D. | AHIN |
| Retinoblastoma | 0.649 | 2.881 | 7.33 | AHIS |
| S100A7 | 0.638 | 0.565 | 2.82 | ALIS |
| Aldolase A | 0.619 | 1.074 | 43.21 | ALIW |
| α-synuclein | 0.619 | 0.926 | 44.93 | ALIW |
| Osteopontin | 0.593 | 3.230 | N.D. | AHIN |
| GST* | 0.530 | 0.415 | 469.5 | ALIW |
| Bcl-2 | 0.372 | 0.708 | 26.83 | ALIW |
| Rac1 | 0.218 | N.D. | 15.41 | ANIW |
| SMAD2 | 0.196 | 0.232 | N.D. | ALIN |
| Fibrinogen | No information | 3.175 | 1.15 | AHIS |
| HSA | No information | 1.356 | N.D. | ALIN |
a β-actin and GSTP1 were used to determine the confidence scores of G-actin and GST from the STRING database.