Fig. 6.

Knockdown of KPNA2 causes subcellular redistribution of E2F1 in lung cancer cells. A, CL1-5 cells were transfected with control siRNA and KPNA2 siRNA, respectively, followed by transfection with E2F1/myc plasmid. At 24 h after transfection, cells transfected with KPNA2 siRNA were mixed 1:1 with control siRNA-transfected cells and re-seeded on coverslips for additional 24 h. At 48 h after transfection, cells were prepared for immunofluorescence staining using anti-KPNA2 and anti-myc antibodies to detect endogenous expression of KPNA2 and exogenous expression of E2F1, respectively. Asterisks indicate KPNA2 knockdown cells. B, Quantification analysis of subcellular distribution acquired from immunofluorescence staining. Error bars indicate standard deviation. A p value of less than 0.05 indicates statistical significance using the unpaired Student's t test. C, CL1-5 cells transfected with control siRNA or KPNA2 siRNA, followed by transfection with E2F1/myc plasmid. At 48 h after transfection, cells were lysed and fractionated as nuclear and cytoplasmic fractions, followed by Western blotting. GAPDH was used as the cytosolic control and lamin A/C as the nuclear control.