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A, HLA-DR and Ii phenotype for parental cells, vaccines, and transfectants. HLA-DR genotype of MCF10 and MCF10A is DR4, DR7. Untransfected cells do not express plasma membrane HLA-DR. B, MCF10 vaccine cells express MHC I, MHC II and CD80, and do not express Invariant chain (Ii). MCF10 parental cells, vaccine cells, and Ii+ MHC II+ positive MCF10 transfectants were stained with antibodies to Ii, HLA-DR, CD80, and HLA-ABC. Data are from 1 of 25 independent experiments.
