Table I. The seven peptides with predicted highest binding affinity to HLA-DR7 efficiently activated PBMC from HLA-DR7⁺ healthy donors.

PBMC activation was assessed as in Fig. 5A–5D. Representative individual experiments from a total of 12 independent experiments. Percent PBMC activation = 100% × (pg IFNγ for experimental peptide/pg IFNγ for Her2 p776). Response >100% indicates priming with the indicated peptide yielded more IFNγ than priming with Her2 p776. * indicates p < 0.05 for experimental peptide vs. p776 as assessed by t test.

ID#	Source	Peptide sequence, ID#, and cell source	Percent PBMC activation relative to Her2 p776
43	Ii−	KVAPAPAVVKKQEAKK	90%
45	Ii−	RTYAGGTASATKVSASSGATSKS	333%*
46	Ii−	SESAAAPAFASSSSEVNPAPKFHW	83%*
47	Ii−	RRMRLTHCGLQEKHL	52%
48	Ii−	GKFYLVIEELSQLFRSLVPIQL	120%
42	Ii− and Ii+	RQTVAVGVIKAVDKKA	96%
44	Ii− and Ii+	ELEEALANASDAELCDIAAILGMHTLM	107%