Fig. 1.

Media conditioned with 293-E4 cells supported survival of primary glomerulus-derived endothelial cells (PrGEC) in the absence of serum and growth factors and increased their life span in complete media. A, Viability of PrGECs cultured for 24 h in complete media or in serum-free media without growth factors with addition of 293 or 293-E4 condition media (1/100 dilution). B, Phase contrast microscopy of PrGECs cultured in complete media with and without 293-E4 condition media for 10 days. Arrows show senescent cells. (Magnification: 200×). C, Growth curve of PrGEC in complete CSC media supplemented with 10% FBS and growth factors with and without the addition of 293-E4 conditioned media. Data are shown as mean ± S.E. from three independent experiments.