Skip to main content
. 2012 Aug 21;11(11):1378–1388. doi: 10.1074/mcp.M112.020313

Fig. 2.

Fig. 2.

293-E4 media supported survival and increased life span of human glomerulus-derived endothelial cells transformed with SV40 large T antigen (HGEC) in the absence of serum and growth factors. A, Expression of SV40 large T antigen in HGEC is shown (black color). Mouse nonspecific antibodies were used as control (Magnification 200×). B, Cell population doubling (PDL) in HGEC with and without 293-E4 condition media. Data are shown as a mean ± STD from 5 independent experiments. C, HGEC at PDL = 25 in the complete media with and without 293-E4 media. (Magnification 200×). D, Cell survival after withdrawal of 293-E4 condition media from HGEC. Arrow shows the day of 293-E4 media withdrawal. Data are shown as a mean ± S.E. from three independent experiments. E, Viability of HGEC cultured for 24 h in complete media or in serum-free media without growth factors with addition of 293 or 293-E4 condition media (1/100 dilution). Data are shown as a mean ± S.E. from three independent experiments.