Fig. 5.

Modeled structures for Cascade subcomplexes based on the cryo-EM density map (20). A dummy-atom filled model was generated from the EM data of the intact complex. Models of the smaller modules were based on the structure of the intact complex, lacking specific subunits, whereby the crRNA (in green) was allowed to rearrange to contact tightly on to the remaining proteins. The other Cas subunits are depicted in the following colors: Cse1, purple; Cse2, yellow; Cas7, gray and blue (for clarity the consecutive Cas7 subunits alternate in color); Cas5, orange; and Cas6e, pink.