Fig. 5.

A1AT prompts migration and invasiveness of lung adenocarcinoma cells. A description of A1AT knockdown and overexpression is provided in Figs. 4A and 4D, respectively. When A1AT was knocked down in CL1-5 cells, the migration and invasiveness of CL1-5 cells was reduced (A, B, and C). A, Cell wound healing assay. The extent of closure was photographed at 0 h and 24 h after treatment at 100× magnification. B, Transwell migration assay at 200× magnification. Migration was quantified by counting cells in six random fields per membrane. C, Matrigel invasion assay at 200× magnification. Invasion was quantified by counting cells in six random fields per membrane. Columns represent the average number of cells per field of at least eighteen fields from three independent experiments. Bars indicate standard deviation. In addition, A1AT was overexpressed in CL1-0 cells to investigate migration and invasiveness. Indeed, the migration and invasiveness of CL1-0 cells was increased in (D) the cell wound healing assay, (E) the transwell migration assay, and (F) the Matrigel invasion assay. All of the data indicate that A1AT protein affects the migration and invasion of lung adenocarcinoma cells. Each assay was performed in independent biological triplicates.