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. 2012 Aug 7;11(11):1263–1273. doi: 10.1074/mcp.M112.017194

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 5. — Senp3 regulates Zbp-89 sumoylation. A–B, Senp3 plays a role in Zbp-89 sumoylation. (A) MEL_BirA (BirA) and MEL_BirA cells expressing bio_HA_Sumo2 (bio_Sumo2) were treated with lentiviruses expressing two different shRNAs against Senp3 (shSenp3 #1, shSenp3 #2) and control lentivirus (shControl). Whole cell lysates (WCL) analyzed by Western blotting with the indicated antibodies. Actin staining serves as a loading control. Nuclear extracts were pull down using magnetic streptavidin beads and analyzed by Western blotting with anti-Zbp-89 antibody. B, MEL_BirA (BirA) and MEL_BirA cells expressing biotinylated Zbp-89 (bio_Zbp-89) were treated with lentiviruses expressing two different shRNAs against Senp3 (shSenp3 #1, shSenp3 #2) and control lentivirus (shControl). Whole cell lysates (WCL) analyzed by Western blotting with the antibodies indicated. Actin staining serves as a loading control. Nuclear extracts were pulled down using magnetic streptavidin beads and analyzed by Western blotting with anti-Zbp-89 and anti Sumo-2/3 antibodies.