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. 2012 Aug 7;11(11):1263–1273. doi: 10.1074/mcp.M112.017194

Fig. 6.

Fig. 6.

5FMC is involved in the regulation of Zbp-89 target genes. A, Pelp1 is recruited at the promoter regions of Dusp6, Zbp-89, Atf5 and the coding region of Tubb1. MEL_BirA cells that ectopically expressed bio_Pelp1 analyzed by ChIP using Pelp1 antibody for the indicated gene promoter or coding regions. The promoter region of the Osm gene and the region upstream of Gata-1 promoter (Gata-1 upstream) were used as negative controls. *** indicates p < 0.001, ** indicates p < 0.01. Error bars: S.D. of triplicate experiment. B, Knockdown of Pelp1, Senp3 and Chtop in MEL_BirA cells. MEL_BirA cells were treated with the indicated shRNAs. Cell lysates were analyzed by Western blotting with the indicated antibodies. Actin staining serves as a loading control. C, Pelp1, Senp3 and Chtop knockdowns reduced RNA polymerase II (Pol II) occupancy at the promoter regions of Dusp6, Zbp-89, Atf5 and the coding region of Tubb1. MEL_BirA cells were treated as in (B). ChIP analysis at the indicated regions was performed using Poll II antibody. Error bars: S.D. of triplicate experiment. D, Regulation of Zbp-89 target genes by Senp3 and Pelp1. Total RNA was extracted from MEL_BirA cells treated as in (B) and analyzed by RT-QPCR for Dusp6, Tubb1, Zbp-89, Atf5, Senp3 and Pelp1. *** indicates p < 0.001, ** indicates p < 0.01, * indicates p < 0.05. Error bars: S.D. of triplicate experiment.