Fig. 5.

EB1 colocalizes with the AIM2 inflammasome and is required for speck-like particle formation in NPC TW02 cells. A, Cells were treated with or without Cy3-labeled plasmids for 2 h, and were further incubated for 4 h. Cells were fixed and were stained with an anti-EB1 antibody, and the colocalization of Cy3-labled plasmids and EB1 was observed by confocal immunofluorescence microscopy. B, and C, Cells were transfected with DsRed-AIM2 or DsRed-ASC for 48 h, treated with poly (dA:dT) for 2 h, and further incubated for 4 h. The cells were then fixed and stained with an anti-EB1 antibody, and the colocalization of EB1 with DsRed-AIM2 or DsRed-ASC was observed by confocal immunofluorescence microscopy. D, NPC TW02 cells expressing control siRNA or EB1 siRNA were cotransfected with GFP-AIM2 and DsRed-ASC for 48 h, treated with or without poly (dA:dT) for 2 h, further incubated for 4 h, and then fixed. The colocalization of GFP-AIM2 and DsRed-ASC was observed by confocal fluorescence microscopy. Scale bar = 20 μm.