Table I. The list of level changed proteins in ASC-pulled down complexes after treatments.
“UN” means untreatment; “-” means no difference after treatment.
| Gene symbol | Accession No. | Protein name | Protein probability | No. of identified peptides | Present coverage % | iTRAQ ratio |
Peptide No. for quantification | Quantitative result |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H2O2/UN (116/114) | S.D. | dAdT/UN (117/114) | S.D. | EBER/UN (115/114) | S.D. | H2O2 | dAdT | EBER | |||||||
| ALB | P02768 | Serum albumin | 1.00 | 8 | 11.7 | 1.21 | 0.40 | 1.06 | 0.27 | 1.04 | 0.41 | 29 | Up | - | - |
| ALS2CR4 | Q96Q45 | Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 4 protein | 1.00 | 4 | 15.3 | 1.31 | 0.23 | 1.06 | 0.23 | 1.11 | 0.24 | 11 | Up | - | - |
| CAV1 | Q03135 | Caveolin-1 | 1.00 | 2 | 9.6 | 0.73 | 0.15 | 0.84 | 0.08 | 0.80 | 0.10 | 7 | Down | - | Down |
| CCT3 | P49368 | T-complex protein 1 subunit gamma | 1.00 | 3 | 6.2 | 0.88 | 0.15 | 0.95 | 0.14 | 0.76 | 0.11 | 6 | - | - | Down |
| CCT6A | P40227 | T-complex protein 1 subunit zeta | 1.00 | 5 | 18.5 | 0.98 | 0.16 | 1.33 | 0.15 | 0.95 | 0.21 | 5 | - | Up | - |
| DHX9 | Q08211 | ATP-dependent RNA helicase A | 1.00 | 3 | 2.0 | 1.07 | 0.09 | 0.93 | 0.06 | 1.43 | 0.20 | 7 | - | - | Up |
| DLST | P36957 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial | 1.00 | 2 | 4.6 | 1.66 | 0.70 | 2.42 | 1.46 | 2.19 | 1.19 | 3 | Up | Up | Up |
| DYNC1H1 | Q14204 | Cytoplasmic dynein 1 heavy chain 1 | 1.00 | 3 | 0.9 | 0.92 | 0.11 | 0.94 | 0.16 | 0.79 | 0.13 | 5 | - | - | Down |
| EB1 (MAPRE1) | Q15691 | End binding protein 1 | 1.00 | 4 | 17.5 | 1.70 | 0.29 | 1.47 | 0.20 | 1.49 | 0.27 | 32 | Up | Up | Up |
| FASN | P49327 | Fatty acid synthase | 1.00 | 9 | 5.7 | 0.74 | 0.19 | 0.97 | 0.15 | 0.95 | 0.17 | 10 | Down | - | - |
| FN1 | P02751 | Fibronectin | 1.00 | 2 | 0.8 | 1.21 | 0.19 | 1.06 | 0.16 | 1.49 | 0.58 | 8 | Up | - | Up |
| GPHN | Q9NQX3 | Gephyrin | 1.00 | 9 | 14.9 | 1.00 | 0.29 | 0.98 | 0.27 | 1.31 | 0.48 | 13 | - | - | Up |
| GRN | P28799 | Granulins | 1.00 | 7 | 16.5 | 1.05 | 0.48 | 0.90 | 0.29 | 0.74 | 0.55 | 27 | - | - | Down |
| H1FX | Q92522 | Histone H1x | 1.00 | 2 | 14.6 | 1.07 | 0.15 | 0.67 | 0.33 | 0.74 | 0.05 | 5 | - | Down | Down |
| HIST1H2AB | P04908 | Histone H2A type 1-B/E | 1.00 | 2 | 21.7 | 1.07 | 0.13 | 1.26 | 0.18 | 1.54 | 0.29 | 4 | - | Up | Up |
| HNRNPA1 | P09651 | Heterogeneous nuclear ribonucleoprotein A1 | 1.00 | 6 | 30.9 | 1.00 | 0.12 | 1.01 | 0.16 | 1.82 | 0.17 | 11 | - | - | Up |
| HNRNPA2B1 | P22626 | Heterogeneous nuclear ribonucleoprotein A2/B1 | 1.00 | 8 | 25.5 | 1.07 | 0.21 | 0.98 | 0.12 | 1.84 | 0.33 | 25 | - | - | Up |
| HNRNPA3 | P51991 | Heterogeneous nuclear ribonucleoprotein A3 | 1.00 | 3 | 16.7 | 0.94 | 0.20 | 0.96 | 0.10 | 1.47 | 0.38 | 6 | - | - | Up |
| HNRNPD | Q14103 | Heterogeneous nuclear ribonucleoprotein D0 | 1.00 | 3 | 12.5 | 1.07 | 0.44 | 1.03 | 0.23 | 1.84 | 1.23 | 11 | - | - | Up |
| HNRNPH1 | P31943 | Heterogeneous nuclear ribonucleoprotein H | 1.00 | 3 | 6.2 | 0.96 | 0.17 | 0.88 | 0.07 | 1.67 | 0.14 | 4 | - | - | Up |
| HNRNPU | Q00839 | Heterogeneous nuclear ribonucleoprotein U | 1.00 | 5 | 7.5 | 1.04 | 0.12 | 1.44 | 0.35 | 1.54 | 0.28 | 4 | - | Up | Up |
| HTRA2 | O43464 | Serine protease HTRA2, mitochondrial | 1.00 | 6 | 21.8 | 1.21 | 0.30 | 0.98 | 0.22 | 1.11 | 0.30 | 10 | Up | - | - |
| IFFO2 | Q5TF58 | Intermediate filament family orphan 2 | 1.00 | 3 | 17.1 | 0.83 | 0.09 | 1.99 | 0.37 | 2.05 | 0.29 | 10 | - | Up | Up |
| KARS | Q15046 | Lysyl-tRNA synthetase | 1.00 | 2 | 2.5 | 0.78 | 0.07 | 0.94 | 0.05 | 0.83 | 0.07 | 10 | Down | - | Down |
| KCTD3 | Q9Y597 | BTB/POX domain-containing protein KCTD3 | 1.00 | 4 | 8.3 | 0.93 | 0.11 | 0.78 | 0.11 | 0.77 | 0.15 | 8 | - | Down | Down |
| LMNA | P02545 | Lamin-A/C | 1.00 | 2 | 3.9 | 0.81 | 0.32 | 0.75 | 0.23 | 0.72 | 0.26 | 3 | - | Down | Down |
| MTHFD1 | P11586 | C-1-tetrahydrofolate synthase, cytoplasmic | 1.00 | 2 | 3.7 | 0.80 | 0.09 | 0.79 | 0.13 | 0.64 | 0.18 | 7 | - | Down | Down |
| MVP | Q14764 | Major vault protein | 1.00 | 11 | 21.2 | 0.86 | 0.24 | 0.72 | 0.24 | 1.56 | 0.68 | 18 | - | Down | Up |
| MYH9 | P35579 | Myosin-9 | 1.00 | 3 | 2.6 | 1.42 | 0.27 | 1.00 | 0.17 | 1.53 | 0.56 | 3 | Up | - | Up |
| MYO1B | O43795 | Myosin-Ib | 1.00 | 3 | 3.3 | 0.78 | 0.13 | 0.84 | 0.11 | 0.70 | 0.22 | 11 | Down | - | Down |
| NCL | P19338 | Nucleolin | 1.00 | 7 | 8.6 | 0.83 | 0.13 | 1.05 | 0.21 | 1.35 | 0.22 | 10 | - | - | Up |
| NIF3L1 | Q9GZT8 | NIF3-like protein 1 | 1.00 | 2 | 4.8 | 1.21 | 0.16 | 1.02 | 0.07 | 1.02 | 0.10 | 7 | Up | - | - |
| NOLC1 | Q14978 | Nucleolar and coiled-body phosphoprotein 1 | 1.00 | 3 | 6.3 | 0.84 | 0.09 | 1.27 | 0.13 | 1.35 | 0.21 | 3 | - | Up | Up |
| NPM1 | P06748 | Nucleophosmin | 1.00 | 3 | 16.1 | 1.04 | 0.20 | 1.25 | 0.17 | 1.88 | 0.29 | 8 | - | Up | Up |
| PCNA | P12004 | Proliferating cell nuclear antigen | 1.00 | 3 | 12.3 | 1.04 | 0.30 | 0.93 | 0.07 | 0.84 | 0.44 | 3 | - | - | Down |
| PHB | P35232 | Prohibitin | 1.00 | 2 | 7.0 | 1.05 | 0.08 | 0.97 | 0.11 | 0.87 | 0.11 | 3 | - | - | Down |
| PHB2 | Q99623 | Prohibitin-2 | 1.00 | 7 | 26.8 | 1.01 | 0.22 | 0.91 | 0.20 | 0.83 | 0.17 | 18 | - | - | Down |
| PKM2 | P14618 | Pyruvate kinase isozymes M1/M2 | 1.00 | 13 | 41.1 | 0.77 | 0.17 | 0.84 | 0.18 | 0.61 | 0.21 | 29 | Down | - | Down |
| PRKDC | P78527 | DNA-dependent protein kinase catalytic subunit | 1.00 | 7 | 3.1 | 0.92 | 0.29 | 0.95 | 0.14 | 0.85 | 0.25 | 13 | - | - | Down |
| PSMC1 | P62191 | 26S protease regulatory subunit 4 | 1.00 | 2 | 7.3 | 0.93 | 0.11 | 0.86 | 0.06 | 0.73 | 0.13 | 4 | - | - | Down |
| RAN | P62826 | GTP-binding nuclear protein Ran | 1.00 | 4 | 25.5 | 0.90 | 0.15 | 0.62 | 0.06 | 0.64 | 0.14 | 9 | - | Down | Down |
| RPL10 | P27635 | 60S ribosomal protein L10 | 1.00 | 3 | 18.7 | 0.86 | 0.06 | 1.28 | 0.13 | 0.94 | 0.05 | 7 | - | Up | - |
| RPL13 | P26373 | 60S ribosomal protein L13 | 1.00 | 3 | 14.2 | 1.14 | 0.08 | 1.29 | 0.17 | 1.28 | 0.24 | 7 | - | Up | - |
| RPL17 | P18621 | 60S ribosomal protein L17 | 1.00 | 3 | 13.6 | 0.72 | 0.11 | 1.15 | 0.25 | 0.97 | 0.16 | 5 | Down | - | - |
| RPL18 | Q07020 | 60S ribosomal protein L18 | 1.00 | 3 | 25.5 | 0.82 | 0.15 | 0.78 | 0.23 | 1.00 | 0.23 | 9 | - | Down | - |
| RPL34 | P49207 | 60S ribosomal protein L34 | 1.00 | 2 | 13.7 | 1.22 | 0.26 | 1.29 | 0.26 | 1.58 | 0.36 | 6 | Up | Up | Up |
| RPL5 | P46777 | 60S ribosomal protein L5 | 1.00 | 4 | 10.8 | 0.95 | 0.07 | 1.03 | 0.22 | 1.29 | 0.17 | 6 | - | - | Up |
| RPS11 | P62280 | 40S ribosomal protein S11 | 1.00 | 5 | 37.3 | 0.90 | 0.13 | 1.01 | 0.13 | 0.82 | 0.12 | 10 | - | - | Down |
| RPS23 | P62266 | 40S ribosomal protein S23 | 1.00 | 2 | 16.1 | 1.17 | 0.19 | 1.26 | 0.14 | 1.22 | 0.19 | 5 | - | Up | - |
| RPS27A | P62988 | Ubiquitin | 1.00 | 2 | 15.8 | 0.86 | 0.04 | 0.88 | 0.16 | 0.78 | 0.07 | 5 | - | - | Down |
| RPS4X | P62701 | 40S ribosomal protein S4, X isoform | 1.00 | 3 | 8.0 | 1.12 | 0.17 | 1.62 | 0.22 | 1.82 | 0.26 | 7 | - | Up | Up |
| RPS7 | P62081 | 40S ribosomal protein S7 | 1.00 | 3 | 19.6 | 0.77 | 0.30 | 0.98 | 0.39 | 0.90 | 0.17 | 3 | Down | - | - |
| RPS9 | P46781 | 40S ribosomal protein S9 | 1.00 | 6 | 25.4 | 0.93 | 0.14 | 1.03 | 0.15 | 0.82 | 0.12 | 8 | - | - | Down |
| SFPQ | P23246 | Splicing factor, proline- and glutamine-rich | 1.00 | 6 | 11.2 | 1.00 | 0.15 | 1.10 | 0.14 | 1.32 | 0.25 | 14 | - | - | Up |
| SLC25A3 | Q00325 | Phosphate carrier protein, mitochondrial | 1.00 | 2 | 5.3 | 1.10 | 0.21 | 1.02 | 0.32 | 0.84 | 0.24 | 5 | - | - | Down |
| SLC25A5 | P05141 | ADP/ATP translocase 2 | 1.00 | 6 | 18.8 | 0.94 | 0.32 | 0.99 | 0.25 | 0.84 | 0.37 | 14 | - | - | Down |
| SMC3 | Q9UQE7 | Structural maintenance of chromosome protein 3 | 1.00 | 2 | 4.6 | 0.74 | 0.15 | 0.83 | 0.11 | 0.83 | 0.03 | 4 | Down | Down | Down |
| TCOF1 | Q13428 | Treacle protein | 1.00 | 2 | 2.1 | 0.99 | 0.06 | 1.19 | 0.17 | 0.86 | 0.08 | 3 | - | - | Down |
| TCP1 | P17987 | T-complex protein 1 subunit alpha | 1.00 | 3 | 5.8 | 1.03 | 0.05 | 0.93 | 0.17 | 0.78 | 0.17 | 5 | - | - | Down |
| TECR | Q9NZ01 | Trans-2,3-enoyl-CoA reductase | 1.00 | 2 | 5.5 | 0.92 | 0.08 | 0.88 | 0.18 | 0.70 | 0.08 | 3 | - | - | Down |
| THBS1 | P07996 | Thrombospondin-1 | 1.00 | 2 | 2.2 | 0.71 | 0.21 | 0.69 | 0.13 | 0.70 | 0.21 | 3 | Down | Down | Down |
| TRIM21 | P19474 | 52 kDa Ro protein | 1.00 | 25 | 57.5 | 1.35 | 0.35 | 1.02 | 0.26 | 1.27 | 0.59 | 113 | UP | - | - |
| TSC1 | Q92574 | Hamartin | 1.00 | 3 | 2.9 | 1.02 | 0.21 | 1.02 | 0.36 | 0.62 | 0.14 | 3 | - | - | Down |
| TTN | Q8WZ42 | Titin | 1.00 | 4 | 0.2 | 0.62 | 0.06 | 0.82 | 0.09 | 0.61 | 0.06 | 180 | Down | Down | Down |
| TUBB | P07437 | Tubulin beta chain | 1.00 | 15 | 45.3 | 0.95 | 0.29 | 0.97 | 0.29 | 0.82 | 0.22 | 42 | - | - | Down |
| TUFM | P49411 | Elongation factor Tu, mitochondrial | 1.00 | 2 | 3.8 | 0.86 | 0.14 | 0.92 | 0.11 | 0.85 | 0.18 | 4 | - | - | Down |
| UQCRC2 | P22695 | Cytochrome b-c1 complex subunit 2, mitochondrial | 1.00 | 3 | 9.1 | 0.92 | 0.34 | 0.81 | 0.24 | 0.88 | 0.53 | 6 | - | Down | - |