Fig. 5.

Serum apoB48 is distributed in smaller LDL and HDL-size particles in Apobec-1^Int/O mice. Following an overnight fast, chow-fed Apobec-1^Int/O and WT mice were administered Pluronic F127 and received an intragastric bolus of lipid. Serum was collected before (time 0) and 4 h after gavage. A: Serum (2 µl) were separated by 4–15% SDS-PAGE and probed with apoB antibody. B: Serum triglyceride levels were evaluated enzymatically at indicated times. Data represent mean ± SE from three WT and six Apobec-1^Int/O mice. C, E: Pooled serum from three WT and six Apobec-1^Int/O animals collected 4 h after lipid gavage was fractionated by FPLC, and 56 (500 µl) fractions were collected. Aliquots of individual fractions were separated by SDS-PAGE and probed with apoB antibody. This is a representative of two separate experiments. Cholesterol (C, E) and triglycerides (D, F) content was enzymatically determined in individual fractions. The reactive bands migrating faster than apoB48 are nonspecific.