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. 2012 Dec;53(12):2560–2572. doi: 10.1194/jlr.M026914

Fig. 5.

Fig. 5.

T-cell (anti-Ova T-cell hybridoma 13.8) stimulating ability of N-BL/6-M ϕ , LD-BL/6 -M ϕ , N-AE-M ϕ , and LD-AE-M ϕ as a function of antigen concentration. (A) The T-cell stimulating ability was assayed in terms of IL-2 production. Data represents mean ± SEM from three different experiments. (B) Quantification of MHC molecule by FACS analysis. Cells were stained with anti-IAb and MFI was presented. Data points are mean ± SD from triplicates. (C) Mobilization of intracellular Ca2+ in T cells. T cells (13.8) were loaded with fluo4-AM and then mixed with ovalbumin pulsed or unpulsed APCs. Synapse formation was assayed in terms of intracellular Ca2+mobilization in T cells. The arrow indicates time of addition of APCs to the fluo4-AM-loaded T cells. BL/6 (upper panel) and AE (lower panel). P, antigen pulsed; UP, antigen unpulsed. Shown is one representative picture from three different experiments. ***P < 0.001, **0.001 < P < 0.01, *0.01 < P < 0.05.