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. 2012 Dec;53(12):2667–2676. doi: 10.1194/jlr.M030734

Fig. 3.

Fig. 3.

Evidence of TOH-ω-hydroxylase activity in microsomes prepared from mouse liver (A–C) and small intestinal mucosa (D–F). GC-MS chromatograms from incubations of liver microsomes from L-Cpr+/+ mice with (A) 250 µM α-TOH, (B) 80 µM γ-TOH, and (C) 80 µM δ-TOH, illustrating formation of the corresponding 13′-OH metabolites. Microsomes from L-Cpr−/− mouse small intestinal mucosa were incubated with (D) 250 µM α-TOH, (E) 80 µM γ-TOH, and (F) 80 µM δ-TOH, illustrating formation of the corresponding 13′-OH metabolites and demonstrating vitamin E-ω-hydroxylase activity in mouse intestine. Retention times and the ratio of the molecular ion to the fragment ion of intestinal samples were consistent with that of the liver.