Fig. 3.

Immunostaining of OSE in a human coronary lesion recognized as pathologic intimal thickening. A: Human coronary lesion shows a superficial plaque with an acellular lipid pool (LP) rich in proteoglycan matrix. Movat pentachrome; I, intima; M, media. B: α-Actin-positive SMCs are mostly localized to the medial wall and superficial layers outside the LP. C: Localization of CD68 staining shows few positive macrophages above the regions of the LP. D: Moderate staining for MD47 is noted for superficial macrophages and deeper areas of the LP. E: MDA2 expression is essentially negative. F: E06 is found primarily localized to superficial macrophages and SMCs above the area of the LP. G: Intense reaction against LPA4 primarily localized to the region of the LP. H: Weak to moderate staining of IK17 is seen in the LP. B–G: Immunoperoxidase (brown reaction product). H: Alkaline phosphatase (red reaction product). Magnification ×200.