TABLE 2.
Top ten of serum metabolite ions that were lower intensity in Smad3-null group with LCA-diet than in wild-type group with LCA-diet
| Rank | Mark | RT (min) | Found (m/z) | Candidate | Mass Error (ppm) |
| 1 | i | 3.19 | 498.2868 | Taurochenodeoxycholate (TCDC) | 4.21 |
| 2 | ii | 3.72 | 482.2913 | Taurolithocholate (TLC) | 5.60 |
| 3 | iii | 3.72 | 480.2764 | Tauro-5β-cholanic acid-3-one (T3KL) | 4.16 |
| 4 | iv | 3.17 | 496.2722 | Not determined | |
| 5 | v | 2.49 | 514.2813 | Tauro-α/β-muricholatea (TαMC/TβMC) | 4.86 |
| 6 | vi | 2.61 | 498.2856 | Tauromurideoxycholate (TMDC) | 6.62 |
| 7 | vii | 3.09 | 498.2860 | Not determined | |
| 8 | viii | 2.77 | 498.2852 | Tauroursodeoxycholate/Taurohyodeoxycholateb (TUDC/THDC) | 7.43 |
| 9 | ix | 2.83 | 496.2700 | Not determined | |
| 10 | x | 2.74 | 496.2703 | Not determined |
The ion ranking, based on the PLS analysis, indicates the highest confidence and greatest contribution to separation between the wild-type and the Smad3-null mice after LCA exposure. RT, retention time.
a
No clear separation between tauro-α-muricholate and tauro-β-muricholate.
b
No clear separation between tauroursodeoxycholate and taurohyodeoxycholate.