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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Nat Cell Biol. 2012 Oct 28;14(11):1223–1230. doi: 10.1038/ncb2593

Figure 5. The C-tail of PARP16 is required for activation of PERK- and IRE1α–mediated UPR.

Figure 5

a, ER microsome based co-immunoprecipitation assays. GFP-PERK or GFP-IRE1α purified from ER microsomes from cells treated with control (Ctrl) or PARP16 (P-16) siRNA, then analysed for BiP binding via immunoblot. PARP16 blots from each assay are shown. b, Immunoblots of cell expressing GFP, or GFP fusions to PARP16 or PARP16Cb5. c, XBP-1 mRNA splicing assay, similar to (b) but performed on PARP16 or PARP16Cb5 overexpressing cells.