(A) H1299, H460, or Saos2 cells were infected with Adhz60 (MOI of 2.5). After 48h, expression of cyclin E was analyzed in cell lysates by Western blot. (B) H1299, H460, or Saos2 cells were cotransduced with Adhz60 (MOI of 2.5) and AdE2F-1, AdE2Ftr, or AdLacz (MOI of 25). After 48h, expression of adenovirus early and late proteins were detected by Western blot. Expression of α-actin was also detected as a loading control. Images are representative of results from three separate experiments.