Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Nat Chem Biol. 2012 Sep 16;8(11):913–919. doi: 10.1038/nchembio.1070

Figure 1.

Figure 1

Discovery of two hydrophobic small molecule-RNA conjugates with [M-H]− m/z = 824.200 and 868.189. (a) Scheme of the general method for discovering biological small molecule-RNA conjugates applied in this work. Total cellular RNA samples are treated with active nuclease P1 or with heat-inactivated nuclease P1 under otherwise identical conditions, then subjected to comparative LC/MS analysis. Nucleotide ions more abundant in the active nuclease sample than in the heat-inactivated nuclease (control) sample represent candidate cellular small molecule-RNA conjugates. (b) Extracted ion chromatogram of AMP-tryptophan and the two unknown nucleotides elucidated in this work.